A theoretical study of polymorphism in VQIVYK fibrils

Jaehoon Yang, Mithila V. Agnihotri, Carol J. Huseby, Jeff Kuret, Sherwin J. Singer

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The VQIVYK fragment from the Tau protein, also known as PHF6, is essential for aggregation of Tau into neurofibrillary lesions associated with neurodegenerative diseases. VQIVYK itself forms amyloid fibrils composed of paired β-sheets. Therefore, the full Tau protein and VQIVYK fibrils have been intensively investigated. A central issue in these studies is polymorphism, the ability of a protein to fold into more than one structure. Using all-atom molecular simulations, we generate five stable polymorphs of VQIVYK fibrils, establish their relative free energy with umbrella sampling methods, and identify the side chain interactions that provide stability. The two most stable polymorphs, which have nearly equal free energy, are formed by interdigitation of the mostly hydrophobic VIY “face” sides of the β-sheets. Another stable polymorph is formed by interdigitation of the QVK “back” sides. When we turn to examine structures from cryo-electron microscopy experiments on Tau filaments taken from diseased patients or generated in vitro, we find that the pattern of side chain interactions found in the two most stable face-to-face as well as the back-to-back polymorphs are recapitulated in amyloid structures of the full protein. Thus, our studies suggest that the interactions stabilizing PHF6 fibrils explain the amyloidogenicity of the VQIVYK motif within the full Tau protein and provide justification for the use of VQIVYK fibrils as a test bed for the design of molecules that identify or inhibit amyloid structures.

Original languageEnglish (US)
Pages (from-to)1396-1416
Number of pages21
JournalBiophysical journal
Issue number8
StatePublished - Apr 20 2021
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics


Dive into the research topics of 'A theoretical study of polymorphism in VQIVYK fibrils'. Together they form a unique fingerprint.

Cite this