TY - JOUR
T1 - A single-cell multi-omic atlas spanning the adult rhesus macaque brain
AU - Cayo Biobank Research Unit
AU - Chiou, Kenneth L.
AU - Huang, Xingfan
AU - Bohlen, Martin O.
AU - Tremblay, Sébastien
AU - DeCasien, Alex R.
AU - O’Day, Diana R.
AU - Spurrell, Cailyn H.
AU - Gogate, Aishwarya A.
AU - Zintel, Trisha M.
AU - Andrews, Madeline G.
AU - Martínez, Melween I.
AU - Starita, Lea M.
AU - Montague, Michael J.
AU - Platt, Michael L.
AU - Shendure, Jay
AU - Snyder-Mackler, Noah
N1 - Publisher Copyright:
© 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
PY - 2023
Y1 - 2023
N2 - Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type–specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions.
AB - Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type–specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions.
UR - http://www.scopus.com/inward/record.url?scp=85174750829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85174750829&partnerID=8YFLogxK
U2 - 10.1126/SCIADV.ADH1914
DO - 10.1126/SCIADV.ADH1914
M3 - Article
C2 - 37824616
AN - SCOPUS:85174750829
SN - 2375-2548
VL - 9
JO - Science Advances
JF - Science Advances
IS - 41
M1 - eadh1914
ER -