A replicable tetrahedral nanostructure self-assembled from a single DNA strand

Zhe Li, Bryan Wei, Jeanette Nangreave, Chenxiang Lin, Yan Liu, Yongli Mi, Hao Yan

Research output: Contribution to journalArticlepeer-review

92 Scopus citations


We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.

Original languageEnglish (US)
Pages (from-to)13093-13098
Number of pages6
JournalJournal of the American Chemical Society
Issue number36
StatePublished - Sep 16 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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