A recirculating-flow fluorescent oxygen sensor

Bruce C. Towe, Scott Flechsig, Glenn Spaulding

Research output: Contribution to journalConference articlepeer-review

6 Scopus citations


An immersible oxygen sensor was constructed by circulating small quantities of ruthenium tris-(2,2'-bipyridyl) II dichloride oxygen-sensitive fluorescent dye through a loop of oxygen-permeable silicone tubing immersed in test medium. The fluorescence intensity of the dye was subsequently measured as it exited the flow loop and related to oxygen tension. This method of measuring the oxygen tension, through diffusive transport to a flowing stream of dye and recirculating it in the sensor, has been found to give a stable response and relatively long sensor lifetime without major recalibration. The sensor showed good stability over at least a week's duration and showed no degradation due to leaching of the dye through membranes or photobleaching that commonly affects fluorescent sensors with immobilized chemistries.

Original languageEnglish (US)
Pages (from-to)799-803
Number of pages5
JournalBiosensors and Bioelectronics
Issue number8
StatePublished - Jan 1 1996
EventProceedings of the 1996 4th World Congress on Biosensors - Bangkok, Thailand
Duration: May 29 1996May 31 1996


  • Fluorescent biosensor
  • Oxygen sensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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