A one-bead one-peptide combinatorial library method for B-cell epitope mapping

Kit S. Lam; Douglas Lake; Sydney E. Salmon; John Smith; Man Ling Chen; Shelly Wade; Farid Abdul-Latif; Richard J. Knapp; Zuzana Leblova; Ronald D. Ferguson; Viktor Krchnak; Nikolai F. Sepetov; Michal Lebl

doi:10.1006/meth.1996.0056

A one-bead one-peptide combinatorial library method for B-cell epitope mapping

Kit S. Lam, Douglas Lake, Sydney E. Salmon, John Smith, Man Ling Chen, Shelly Wade, Farid Abdul-Latif, Richard J. Knapp, Zuzana Leblova, Ronald D. Ferguson, Viktor Krchnak, Nikolai F. Sepetov, Michal Lebl

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide-beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-β-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) antihuman insulin (discontinuous epitope), and (iv) surface immunoglobulins (μκ) of two murine B-cell lymphoma cell lines (antigen unknown).

Original language	English (US)
Pages (from-to)	482-493
Number of pages	12
Journal	Methods: A Companion to Methods in Enzymology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1006/meth.1996.0056
State	Published - Jun 1996
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1006/meth.1996.0056

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title = "A one-bead one-peptide combinatorial library method for B-cell epitope mapping",

abstract = "The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide-beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-β-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) antihuman insulin (discontinuous epitope), and (iv) surface immunoglobulins (μκ) of two murine B-cell lymphoma cell lines (antigen unknown).",

author = "Lam, {Kit S.} and Douglas Lake and Salmon, {Sydney E.} and John Smith and Chen, {Man Ling} and Shelly Wade and Farid Abdul-Latif and Knapp, {Richard J.} and Zuzana Leblova and Ferguson, {Ronald D.} and Viktor Krchnak and Sepetov, {Nikolai F.} and Michal Lebl",

note = "Funding Information: This work is supported by NIH Grants CA17094, CA23074, and CA57723 and by Selectide Corporation. Kit S. Lam is a Scholar of the Leukemia Society of America. We thank Ms. Cindy Ryan for secretarial support.",

year = "1996",

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doi = "10.1006/meth.1996.0056",

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AU - Lam, Kit S.

AU - Lake, Douglas

AU - Salmon, Sydney E.

AU - Smith, John

AU - Chen, Man Ling

AU - Wade, Shelly

AU - Abdul-Latif, Farid

AU - Knapp, Richard J.

AU - Leblova, Zuzana

AU - Ferguson, Ronald D.

AU - Krchnak, Viktor

AU - Sepetov, Nikolai F.

AU - Lebl, Michal

N1 - Funding Information: This work is supported by NIH Grants CA17094, CA23074, and CA57723 and by Selectide Corporation. Kit S. Lam is a Scholar of the Leukemia Society of America. We thank Ms. Cindy Ryan for secretarial support.

PY - 1996/6

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A one-bead one-peptide combinatorial library method for B-cell epitope mapping

Abstract

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