A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

J. P.J. Chen; K. E. Schmidt; J. C.H. Spence; R. A. Kirian

doi:10.1016/j.ultramic.2021.113234

A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

J. P.J. Chen, K. E. Schmidt, J. C.H. Spence, R. A. Kirian

Physics

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9 Scopus citations

Abstract

We develop an algorithm capable of imaging a three-dimensional object given a collection of two-dimensional images of that object that are significantly influenced by the curvature of the Ewald sphere. These two-dimensional images cannot be approximated as projections of the object. Such an algorithm is useful in cryo-electron microscopy where larger samples, higher resolution, or lower energy electron beams are desired, all of which contribute to the significance of Ewald curvature.

Original language	English (US)
Article number	113234
Journal	Ultramicroscopy
Volume	224
DOIs	https://doi.org/10.1016/j.ultramic.2021.113234
State	Published - May 2021

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Instrumentation

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10.1016/j.ultramic.2021.113234

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abstract = "We develop an algorithm capable of imaging a three-dimensional object given a collection of two-dimensional images of that object that are significantly influenced by the curvature of the Ewald sphere. These two-dimensional images cannot be approximated as projections of the object. Such an algorithm is useful in cryo-electron microscopy where larger samples, higher resolution, or lower energy electron beams are desired, all of which contribute to the significance of Ewald curvature.",

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AU - Schmidt, K. E.

AU - Spence, J. C.H.

AU - Kirian, R. A.

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A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

Abstract

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