A new approach for measuring single-cell oxygen consumption rates

Timothy W. Molter, Mark R. Holl, Joseph M. Dragavon, Sarah C. McQuaide, Judith B. Anderson, A. Cody Young, Lloyd W. Burgess, Mary E. Lidstrom, Deirdre Meldrum

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalIEEE Transactions on Automation Science and Engineering
Issue number1
StatePublished - Jan 2008


  • Biochemistry
  • Frequency-domain analysis
  • Image processing
  • Oxygen
  • Phosphorus compounds

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering


Dive into the research topics of 'A new approach for measuring single-cell oxygen consumption rates'. Together they form a unique fingerprint.

Cite this