A Modified Uridine in the Anticodon of E. Coli

S. Altman

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalNucleic acids research
Issue number2
StatePublished - Feb 1976
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


Dive into the research topics of 'A Modified Uridine in the Anticodon of E. Coli'. Together they form a unique fingerprint.

Cite this