A Highly Selective Mitochondria-Targeting Fluorescent K+ Sensor

Xiangxing Kong; Fengyu Su; Liqiang Zhang; Jordan Yaron; Fred Lee; Zhengwei Shi; Yanqing Tian; Deirdre Meldrum

doi:10.1002/anie.201506038

A Highly Selective Mitochondria-Targeting Fluorescent K⁺ Sensor

Xiangxing Kong, Fengyu Su, Liqiang Zhang, Jordan Yaron, Fred Lee, Zhengwei Shi, Yanqing Tian, Deirdre Meldrum

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Regulation of intracellular potassium (K⁺) concentration plays a key role in metabolic processes. So far, only a few intracellular K⁺ sensors have been developed. The highly selective fluorescent K⁺ sensor KS6 for monitoring K⁺ ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K⁺ in the range 30-500 mM, a large dynamic range (F_max/F₀≈130), high brightness (φ_f=14.4 % at 150 mM of K⁺), and insensitivity to both pH in the range 5.5-9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K⁺ efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K⁺ sensor suitable for the study of mitochondrial potassium flux in live cells.

Original language	English (US)
Pages (from-to)	12053-12057
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	41
DOIs	https://doi.org/10.1002/anie.201506038
State	Published - Oct 1 2015

Keywords

BODIPY
fluorescent probes
ion channels
mitochondria
potassium

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Access to Document

10.1002/anie.201506038

Cite this

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title = "A Highly Selective Mitochondria-Targeting Fluorescent K+ Sensor",

abstract = "Regulation of intracellular potassium (K+) concentration plays a key role in metabolic processes. So far, only a few intracellular K+ sensors have been developed. The highly selective fluorescent K+ sensor KS6 for monitoring K+ ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K+ in the range 30-500 mM, a large dynamic range (Fmax/F0≈130), high brightness (φf=14.4 % at 150 mM of K+), and insensitivity to both pH in the range 5.5-9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K+ efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K+ sensor suitable for the study of mitochondrial potassium flux in live cells.",

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AU - Shi, Zhengwei

AU - Tian, Yanqing

AU - Meldrum, Deirdre

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