3D Framework DNA Origami with Layered Crossovers

Fan Hong, Shuoxing Jiang, Tong Wang, Yan Liu, Hao Yan

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Designer DNA architectures with nanoscale geometric controls provide a programmable molecular toolbox for engineering complex nanodevices. Scaffolded DNA origami has dramatically improved our ability to design and construct DNA nanostructures with finite size and spatial addressability. Here we report a novel design strategy to engineer multilayered wireframe DNA structures by introducing crossover pairs that connect neighboring layers of DNA double helices. These layered crossovers (LX) allow the scaffold or helper strands to travel through different layers and can control the relative orientation of DNA helices in neighboring layers. Using this design strategy, we successfully constructed four versions of two-layer parallelogram structures with well-defined interlayer angles, a three-layer structure with triangular cavities, and a 9- and 15-layer square lattices. This strategy provides a general route to engineer 3D framework DNA nanostructures with controlled cavities and opportunities to design host–guest networks analogs to those produced with metal organic frameworks.

Original languageEnglish (US)
Pages (from-to)12832-12835
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number41
StatePublished - Oct 4 2016


  • DNA nanotechnology
  • DNA origami structures
  • framework structures
  • molecular programming
  • self-assembly

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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