Serial femtosecond crystallography datasets from G protein-coupled receptors

  • Anton Barty (Contributor)
  • Wei Liu (Contributor)
  • Andrii Ishchenko (Contributor)
  • Haitao Zhang (Contributor)
  • Cornelius Gati (Contributor)
  • Nadia A. Zatsepin (Contributor)
  • Shibom Basu (Contributor)
  • Dominik Oberthür (Contributor)
  • Markus Metz (Contributor)
  • Kenneth Beyerlein (Contributor)
  • Chun Hong Yoon (Contributor)
  • Oleksandr Yefanov (Contributor)
  • Daniel James (Contributor)
  • Dingjie Wang (Contributor)
  • Marc Messerschmidt (Contributor)
  • Jason E. Koglin (Contributor)
  • Sébastien Boutet (Contributor)
  • Uwe Weierstall (Contributor)
  • Vadim Cherezov (Contributor)



Scientific data 3, 160057 (2016). doi:10.1038/sdata.2016.57 special issue: "Structural biology applications of X-ray lasers",,We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2 , the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.,Published by Nature Publ. Group, London,
Date made availableJan 1 2016
PublisherDESY - Deutsches Elektronen-Synchrotron

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