Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes

  • Samantha E. Day (Creator)
  • Richard L. Coletta (Creator)
  • Joon Young Kim (Creator)
  • Latoya E. Campbell (Contributor)
  • Tonya R. Benjamin (Creator)
  • Lori R. Roust (Creator)
  • Elena A. De Filippis (Contributor)
  • Valentin Dinu (Creator)
  • Gabriel Shaibi (Creator)
  • Lawrence J. Mandarino (Creator)
  • Dawn K. Coletta (Mayo Clinic Scottsdale AZ, Arizona State University) (Creator)



Abstract Background Obesity is a metabolic disease caused by environmental and genetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to investigate the role of skeletal muscle DNA methylation in combination with transcriptomic changes in obesity. Results Muscle biopsies were obtained basally from lean (n = 12; BMI = 23.4 ± 0.7 kg/m2) and obese (n = 10; BMI = 32.9 ± 0.7 kg/m2) participants in combination with euglycemic-hyperinsulinemic clamps to assess insulin sensitivity. We performed reduced representation bisulfite sequencing (RRBS) next-generation methylation and microarray analyses on DNA and RNA isolated from vastus lateralis muscle biopsies. There were 13,130 differentially methylated cytosines (DMC; uncorrected P 
Date made available2016
Publisherfigshare Academic Research System

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