In-solution Y-chromosome capture-enrichment on ancient DNA libraries

  • Diana I. Cruz-Dávalos (Creator)
  • Maria A. Nieves-Colón (Creator)
  • Alexandra Sockell (Creator)
  • G. David Poznik (Contributor)
  • Hannes Schroeder (Creator)
  • Anne Stone (Creator)
  • Carlos D. Bustamante (Creator)
  • Anna Sapfo Malaspinas (Contributor)
  • María C. Ávila-Arcos (Creator)
  • Carlos D. Bustamante (Creator)
  • Malaspinas Anna-Sapfo (Contributor)



Abstract Background As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach—in-solution capture-enrichment—retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC + YCC) versus non-enriched (pre-capture) libraries. Results The six samples show different levels of initial endogenous content, with very low (
Date made available2018

Cite this