Additional file 7: HISAT mapped reads bar plot. Mean difference in expression for average total reads mapped for each tissue and each sex when aligned to a sex chromosome informed versus a default reference genome. Paired t-test to test for significant difference in total reads mapped for the whole transcriptome, chromosome 8, and chromosome X. Nonparametric Wilcox single rank sum test was used to test for significant difference in total reads mapped on the Y chromosome for male samples in each tissue separately. Red * indicate a significant, p-value <0.05, difference in average mapped reads, NS is no significant differences.