Additional file 1 of Genome-wide investigation of maize RAD51 binding affinity through phage display

  • Claire Milsted (Creator)
  • Bo Dai (Creator)
  • Nelson Garcia (Creator)
  • Lu Yin (Creator)
  • Yan He (Creator)
  • Shahryar Kianian (Creator)
  • Wojciech P. Pawlowski (Creator)
  • Changbin Chen (Creator)



Additional file 1: Figure S1. ELISA screening of phages with native and denatured RAD51A1 proteins. Wells with an OD450 1.5-fold or more above the BSA control were coded as positive. Phages 4, 5, 6, 7, 14, 15, 16, 19, 20, 21, 22 and 23 were positive against both native and denatured protein. Phages 9, 10, 13, and 17 were positive against denatured protein only. Figure S2. Phage dot blotting with native RAD51A1. (1) supernatant (2) pellet (3) flow-through (4) first elution and (5) second elution. Phages 5,8,13,14, and 20 bound to the protein supernatant, protein pellet, flow-through, as well as the first elution. This could be due to RAD51A1 present in these stages of the purification process, or due to the fact that the earlier stages of the purification process would contain E. coli host proteins and these phages which live in E. coli may interact with E. coli proteins. High imidazole concentration may have inhibited localization of the protein on the membrane in the second elution, resulting in false negatives. Table S1. Proteins from non-maize species with alignments to selected phage peptides in Table 1. Figure S3. Dot blotting of 32 synthesized peptides with RAD51A1 on nitrocellulose. Amino acid sequences of all 32 peptides are listed in Table 3. Some peptides were blotted twice. Peptides 1, 2, 3, 7, 8, 11, 12, 15, 18, 19, 20, 25, 30, and 31 bound to RAD51A1. These 14 peptides are listed in Table 4. Note: Two dot blotting experiments were conducted due to the availability of synthesized peptides, GenScript provided two batches of peptide products
Date made available2022

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