Additional file 1: Fig. S1. Transfection efficiency is not predictive of editing efficiency. HEK293 cells were transfected with pEF-mCherry, pCMV-ABEmax, and sg(TS). Comparison of transfection efficiency (percentage of mCherry-positive cells) and editing efficiency (percentage of A-to-G conversion at target nucleotides) in unsorted cell populations targeted at various genomic loci.
Brafman, D. A. (Contributor), Nguyen, T. (Contributor), Standage-Beier, K. (Contributor), Tekel, S. J. (Contributor), Wang, X. (Contributor), Brookhouser, N. (Contributor) (Jan 1 2020). Additional file 1 of A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells. figshare Academic Research System. 10.6084/m9.figshare.13467907.v1