3GYR : Structure of Phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center.

  • A. W. Smith (Contributor)
  • A. C├ímara-Artigas (Contributor)
  • Meitian Wang (Contributor)
  • James Allen (Contributor)
  • Wilson A. Francisco (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2009-04-28
Deposition Date:2009-04-05
Revision Date:2011-07-13
Molecular Weight:810238.94
Macromolecule Type:Protein
Residue Count:7344
Atom Site Count:54864

The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
Date made available2009

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