Titanium Dioxide Nanoparticles in Advanced Imaging and Nanotherapeutics

Tijana Rajh, Nada M. Dimitrijevic, Elena A. Rozhkova

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations


Semiconductor photocatalysis using nanoparticulate TiO2 has proven to be a promising technology for use in catalytic reactions, in the cleanup of water contaminated with hazardous industrial by-products, and in nanocrystalline solar cells as a photoactive material. Metal oxide semiconductor colloids are of considerable interest because of their photocatalytic properties. The coordination sphere of the surface metal atoms is incomplete and thus traps light-induced charges, but also exhibits high affinity for oxygencontaining ligands and gives the opportunity for chemical modification. We use enediol linkers, such as dopamine and its analogs, to bridge the semiconductors to biomolecules such as DNA or proteins. Nanobio hybrids that combine the physical robustness and chemical reactivity of nanoscale metal oxides with the molecular recognition and selectivity of biomolecules were developed. Control of chemical processes within living cells was achieved using TiO2 nanocomposites in order to develop new tools for advanced nanotherapeutics. Here, we describe general experimental approaches for synthesis and characterization of high crystallinity, water soluble 5 nm TiO2 particles and their nanobio composites, methods of cellular sample preparation for advanced Synchrotron-based imaging of nanoparticles in single cell X-ray fluorescence, and a detailed experimental setup for application of the high-performance TiO2-based nanobio photocatalyst for targeted lysis of cancerous or other disordered cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Antibody
  • Charge transfer complex
  • DNA
  • Hybrid composites
  • Nanoparticles
  • Photocatalysis
  • Surface reconstruction
  • Synchrotron X-ray fluorescence
  • Targeted cancer therapy
  • TiO

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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