TY - JOUR
T1 - The glyphosate-based herbicide roundup does not elevate genome-wide mutagenesis of Escherichia coli
AU - Tincher, Clayton
AU - Long, Hongan
AU - Behringer, Megan
AU - Walker, Noah
AU - Lynch, Michael
N1 - Funding Information:
We thank Chloe Strauss, Samuel Miller, Caitlyn Patterson, Colin Gregory, and Thomas G. Doak for technical help. This research is supported by the Multidisciplinary University Research Initiative Award W911NF-09-1-0444 from the US Army Research Office and National Institutes of Health grant R01 GM036827.
Publisher Copyright:
© 2017 Tincher et al.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
AB - Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
KW - Ecological dependence of mutations
KW - Environmental mutagenesis
KW - Evolutionary genomics
KW - Herbicide damage
KW - Mutagenicity test
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U2 - 10.1534/g3.117.300133
DO - 10.1534/g3.117.300133
M3 - Article
C2 - 28983068
AN - SCOPUS:85030624190
SN - 2160-1836
VL - 7
SP - 3331
EP - 3335
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
IS - 10
ER -