The cryo-EM method microcrystal electron diffraction (MicroED)

Brent Nannenga, Tamir Gonen

Research output: Contribution to journalReview articlepeer-review

147 Scopus citations


In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.

Original languageEnglish (US)
Pages (from-to)369-379
Number of pages11
JournalNature Methods
Issue number5
StatePublished - May 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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