Teflon ™-coated silicon apertures for supported lipid bilayer membranes

S. J. Wilk; Michael Goryll; G. M. Laws; Stephen Goodnick; Trevor Thornton; Marco Saraniti; J. Tang; R. S. Eisenberg

doi:10.1063/1.1805712

Teflon ™-coated silicon apertures for supported lipid bilayer membranes

S. J. Wilk, Michael Goryll, G. M. Laws, Stephen Goodnick, Trevor Thornton, Marco Saraniti, J. Tang, R. S. Eisenberg

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

We present a method for microfabricating apertures in a silicon substrate using well-known cleanroom technologies resulting in highly reproducible giga-seal resistance bilayer formations. Using a plasma etcher, 150 μm apertures have been etched through a silicon wafer. Teflon™ has been chemically vapor deposited so that the surface resembles bulk Teflon and is hydrophobic. After fabrication, reproducible high resistance bilayers were formed and characteristic measurements of a self-inserted single OmpF porin ion channel protein were made.

Original language	English (US)
Pages (from-to)	3307-3309
Number of pages	3
Journal	Applied Physics Letters
Volume	85
Issue number	15
DOIs	https://doi.org/10.1063/1.1805712
State	Published - Oct 11 2004

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.1805712

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abstract = "We present a method for microfabricating apertures in a silicon substrate using well-known cleanroom technologies resulting in highly reproducible giga-seal resistance bilayer formations. Using a plasma etcher, 150 μm apertures have been etched through a silicon wafer. Teflon{\texttrademark} has been chemically vapor deposited so that the surface resembles bulk Teflon and is hydrophobic. After fabrication, reproducible high resistance bilayers were formed and characteristic measurements of a self-inserted single OmpF porin ion channel protein were made.",

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AU - Wilk, S. J.

AU - Goryll, Michael

AU - Laws, G. M.

AU - Goodnick, Stephen

AU - Thornton, Trevor

AU - Saraniti, Marco

AU - Tang, J.

AU - Eisenberg, R. S.

PY - 2004/10/11

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AB - We present a method for microfabricating apertures in a silicon substrate using well-known cleanroom technologies resulting in highly reproducible giga-seal resistance bilayer formations. Using a plasma etcher, 150 μm apertures have been etched through a silicon wafer. Teflon™ has been chemically vapor deposited so that the surface resembles bulk Teflon and is hydrophobic. After fabrication, reproducible high resistance bilayers were formed and characteristic measurements of a self-inserted single OmpF porin ion channel protein were made.

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Teflon ™-coated silicon apertures for supported lipid bilayer membranes

Abstract

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