@inbook{25e6f59a82bf4ba9af55c02e55375792,
title = "T-cell epitope discovery for therapeutic cancer vaccines",
abstract = "The success of recent immune checkpoint blockade trials in solid tumors has demonstrated the tremendous potential of immune-mediated treatment strategies for cancer therapy. These immune therapies activate preexisting cytotoxic CD8+ T cells (CTL) to selectively target and eradicate malignant cells. In vitro models suggest that these therapies may be more effective in combination with priming of CTL using cancer vaccines. CTL-mediated tumor targeting is achieved by its recognition of tumor antigenic epitopes presented on human leukocyte antigen (HLA) class I molecules by tumor cells. Discovering CTL-antigenic epitopes is therefore central to the design of therapeutic T-cell vaccines and immune monitoring of these complex immunotherapies. However, selecting and monitoring T-cell epitopes remains difficult due to the extensive polymorphism of HLA alleles and the presence of confounding non-immunogenic self-peptides. To overcome these challenges, this chapter presents methodologies for the design of CTL-targeted vaccines using selection of target HLA alleles, novel integrated computational strategies to predict HLA-class I CTL epitopes, and epitope validation methods using short-term ex vivo T-cell stimulation. This strategy results in the improved efficiency for selecting antigenic epitopes for CTL-mediated vaccines and for immune monitoring of tumor antigens.",
keywords = "Cytotoxic CD8 T cells, HLA typing, T-cell epitope",
author = "Sri Krishna and Karen Anderson",
note = "Funding Information: We thank Diego Chowell for assistance with the normalization of prediction algorithms and epitope discovery strategy. This chapter describes work supported by institutional funds from Arizona State University to S.K. and K.S.A. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = apr,
day = "1",
doi = "10.1007/978-1-4939-3387-7_45",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "779--796",
booktitle = "Methods in Molecular Biology",
}