Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson; David Arnlund; Gergely Katona; Thomas A. White; Anton Barty; Daniel P. Deponte; Robert L. Shoeman; Cecilia Wickstrand; Amit Sharma; Garth J. Williams; Andrew Aquila; Michael J. Bogan; Carl Caleman; Jan Davidsson; R. Bruce Doak; Matthias Frank; Raimund Fromme; Lorenzo Galli; Ingo Grotjohann; Mark S. Hunter; Stephan Kassemeyer; Richard Kirian; Christopher Kupitz; Mengning Liang; Lukas Lomb; Erik Malmerberg; Andrew V. Martin; Marc Messerschmidt; Karol Nass; Lars Redecke; M. Marvin Seibert; Jennie Sjöhamn; Jan Steinbrener; Francesco Stellato; Dingjie Wang; Weixaio Y. Wahlgren; Uwe Weierstall; Sebastian Westenhoff; Nadia Zatsepin; Sébastien Boutet; John Spence; Ilme Schlichting; Henry N. Chapman; Petra Fromme; Richard Neutze

doi:10.1038/ncomms3911

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Gergely Katona, Thomas A. White, Anton Barty, Daniel P. Deponte, Robert L. Shoeman, Cecilia Wickstrand, Amit Sharma, Garth J. Williams, Andrew Aquila, Michael J. Bogan, Carl Caleman, Jan Davidsson, R. Bruce Doak, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S. HunterStephan Kassemeyer, Richard Kirian, Christopher Kupitz, Mengning Liang, Lukas Lomb, Erik Malmerberg, Andrew V. Martin, Marc Messerschmidt, Karol Nass, Lars Redecke, M. Marvin Seibert, Jennie Sjöhamn, Jan Steinbrener, Francesco Stellato, Dingjie Wang, Weixaio Y. Wahlgren, Uwe Weierstall, Sebastian Westenhoff, Nadia Zatsepin, Sébastien Boutet, John Spence, Ilme Schlichting, Henry N. Chapman, Petra Fromme, Richard Neutze

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Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Original language	English (US)
Article number	2911
Journal	Nature communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms3911
State	Published - Dec 19 2013

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Johansson, L. C., Arnlund, D., Katona, G., White, T. A., Barty, A., Deponte, D. P., Shoeman, R. L., Wickstrand, C., Sharma, A., Williams, G. J., Aquila, A., Bogan, M. J., Caleman, C., Davidsson, J., Doak, R. B., Frank, M., Fromme, R., Galli, L., Grotjohann, I., ... Neutze, R. (2013). Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. Nature communications, 4, Article 2911. https://doi.org/10.1038/ncomms3911

Johansson, LC, Arnlund, D, Katona, G, White, TA, Barty, A, Deponte, DP, Shoeman, RL, Wickstrand, C, Sharma, A, Williams, GJ, Aquila, A, Bogan, MJ, Caleman, C, Davidsson, J, Doak, RB, Frank, M, Fromme, R, Galli, L, Grotjohann, I, Hunter, MS, Kassemeyer, S, Kirian, R, Kupitz, C, Liang, M, Lomb, L, Malmerberg, E, Martin, AV, Messerschmidt, M, Nass, K, Redecke, L, Seibert, MM, Sjöhamn, J, Steinbrener, J, Stellato, F, Wang, D, Wahlgren, WY, Weierstall, U, Westenhoff, S, Zatsepin, N, Boutet, S, Spence, J, Schlichting, I, Chapman, HN, Fromme, P & Neutze, R 2013, 'Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography', Nature communications, vol. 4, 2911. https://doi.org/10.1038/ncomms3911

@article{5ca2dad906094ddb99212ac9689f201f,

title = "Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography",

abstract = "Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 {\AA} resolution and determine its serial femtosecond crystallography structure to 3.5 {\AA} resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.",

author = "Johansson, {Linda C.} and David Arnlund and Gergely Katona and White, {Thomas A.} and Anton Barty and Deponte, {Daniel P.} and Shoeman, {Robert L.} and Cecilia Wickstrand and Amit Sharma and Williams, {Garth J.} and Andrew Aquila and Bogan, {Michael J.} and Carl Caleman and Jan Davidsson and Doak, {R. Bruce} and Matthias Frank and Raimund Fromme and Lorenzo Galli and Ingo Grotjohann and Hunter, {Mark S.} and Stephan Kassemeyer and Richard Kirian and Christopher Kupitz and Mengning Liang and Lukas Lomb and Erik Malmerberg and Martin, {Andrew V.} and Marc Messerschmidt and Karol Nass and Lars Redecke and Seibert, {M. Marvin} and Jennie Sj{\"o}hamn and Jan Steinbrener and Francesco Stellato and Dingjie Wang and Wahlgren, {Weixaio Y.} and Uwe Weierstall and Sebastian Westenhoff and Nadia Zatsepin and S{\'e}bastien Boutet and John Spence and Ilme Schlichting and Chapman, {Henry N.} and Petra Fromme and Richard Neutze",

note = "Funding Information: Support for LLNL personnel was provided by the UCOP Lab Fee Program (award number 118036) and the LLNL Lab-directed Research and Development Program (12-ERD-031). Funding Information: Experiments were carried out at the LCLS, a national user facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences. We gratefully acknowledge financial support from the Swedish Science Research Council (VR), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), the Swedish Strategic Research Foundation (SSF), the US National Science Foundation (NSF) and its bioXFEL Science and Technology Center (NSF 1231306), the US National Institute of Health (NIH), the DOE Office of Basic Energy Sciences, the Hamburg Ministry of Science and Research, the Joachim Herz Stiftung, the Deutsche Forschungsgemeinschaft (DFG), the German Federal Ministry for Education and Research (BMBF), the DFG Cluster of Excellence {\textquoteleft}Inflammation at Interfaces{\textquoteright} (EXC 306) and the Max Planck Society.",

year = "2013",

month = dec,

day = "19",

doi = "10.1038/ncomms3911",

language = "English (US)",

volume = "4",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

AU - Johansson, Linda C.

AU - Arnlund, David

AU - Katona, Gergely

AU - White, Thomas A.

AU - Barty, Anton

AU - Deponte, Daniel P.

AU - Shoeman, Robert L.

AU - Wickstrand, Cecilia

AU - Sharma, Amit

AU - Williams, Garth J.

AU - Aquila, Andrew

AU - Bogan, Michael J.

AU - Caleman, Carl

AU - Davidsson, Jan

AU - Doak, R. Bruce

AU - Frank, Matthias

AU - Fromme, Raimund

AU - Galli, Lorenzo

AU - Grotjohann, Ingo

AU - Hunter, Mark S.

AU - Kassemeyer, Stephan

AU - Kirian, Richard

AU - Kupitz, Christopher

AU - Liang, Mengning

AU - Lomb, Lukas

AU - Malmerberg, Erik

AU - Martin, Andrew V.

AU - Messerschmidt, Marc

AU - Nass, Karol

AU - Redecke, Lars

AU - Seibert, M. Marvin

AU - Sjöhamn, Jennie

AU - Steinbrener, Jan

AU - Stellato, Francesco

AU - Wang, Dingjie

AU - Wahlgren, Weixaio Y.

AU - Weierstall, Uwe

AU - Westenhoff, Sebastian

AU - Zatsepin, Nadia

AU - Boutet, Sébastien

AU - Spence, John

AU - Schlichting, Ilme

AU - Chapman, Henry N.

AU - Fromme, Petra

AU - Neutze, Richard

N1 - Funding Information: Support for LLNL personnel was provided by the UCOP Lab Fee Program (award number 118036) and the LLNL Lab-directed Research and Development Program (12-ERD-031). Funding Information: Experiments were carried out at the LCLS, a national user facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences. We gratefully acknowledge financial support from the Swedish Science Research Council (VR), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), the Swedish Strategic Research Foundation (SSF), the US National Science Foundation (NSF) and its bioXFEL Science and Technology Center (NSF 1231306), the US National Institute of Health (NIH), the DOE Office of Basic Energy Sciences, the Hamburg Ministry of Science and Research, the Joachim Herz Stiftung, the Deutsche Forschungsgemeinschaft (DFG), the German Federal Ministry for Education and Research (BMBF), the DFG Cluster of Excellence ‘Inflammation at Interfaces’ (EXC 306) and the Max Planck Society.

PY - 2013/12/19

Y1 - 2013/12/19

N2 - Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

AB - Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

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U2 - 10.1038/ncomms3911

DO - 10.1038/ncomms3911

M3 - Article

C2 - 24352554

AN - SCOPUS:84890950819

SN - 2041-1723

VL - 4

JO - Nature communications

JF - Nature communications

M1 - 2911

ER -

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Abstract

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