Structure and function of glucose binding protein-single walled carbon nanotube complexes

Thomas P. McNicholas, Kyungsuk Yum, Jin Ho Ahn, Bin Mu, Oliver Plettenburg, Annlouise Gooderman, Sridaran Natesan, Michael S. Strano

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Understanding the structure and function of glucose binding proteins (GBP) complexed with single walled carbon nanotubes (SWNTs) is important for the development of applications including fluorescent sensors and nanostructure particle tracking. Herein, circular dichroism (CD), thermal denaturation, photo-absorption spectroscopy and atomic force microscopy are used to study these nanostructures. The protein retains its glucose-binding activity after complexation and is thermally stable below 36 °C. However, the SWNT lowers the midpoint denaturation temperature (Tm) by 5°C and 4°C in the absence and presence of 10 mM glucose, respectively. This data highlights that using techniques such as CD and thermal denaturation may be necessary to fully characterize such protein-nanomaterial nanostructures.

Original languageEnglish (US)
Pages (from-to)3510-3516
Number of pages7
Issue number22
StatePublished - Nov 19 2012
Externally publishedYes


  • carbon nanotubes
  • fluorescence
  • glucose
  • periplasmic-binding proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)


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