Abstract
We report a miniature separator for medical microdevices. The separator uses a size-exclusion chromatography (SEC) mini-column in liquid chromatography (LC). The mini-column is fabricated on a glass slide with poly- dimethylsiloxane (PDMS) for structure. Polydivinlybenzene nanobeads (5-20mm in diameter, 100-nm pore size) were used. The SEC mini-column successfully separates a-fetoprotein (AFP) and immunoglobulin G (IgG) at 5 and 9min, respectively, with 0.86 resolution and 0.06-mm plate height. The mini-column shows potential for use in onboard sample preparation in a total analysis system (mTAS) for a point-of-care testing (POCT) device that could be used effectively in cancer screening, diagnosis, and prognosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1328-1340 |
| Number of pages | 13 |
| Journal | Analytical Letters |
| Volume | 42 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2009 |
Keywords
- A-fetoprotein
- Cancer biomarker
- Liquid chromatography
- MEMS
- Separation
- Size-exclusion chromatography
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Spectroscopy
- Clinical Biochemistry
- Biochemistry, medical
- Electrochemistry
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