Real-time monitoring of phosphorylation kinetics with self-assembled nano-oscillators

Yimin Fang, Shan Chen, Wei Wang, Xiaonan Shan, Nongjian Tao

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.

Original languageEnglish (US)
Pages (from-to)2538-2542
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number8
StatePublished - Feb 16 2015


  • Charge-based detection
  • Nano-oscillators
  • Phosphorylation
  • Plasmonic imaging
  • Real-time imaging

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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