@inproceedings{c421ac099d54455fac9436c38eac2ff5,
title = "Radical activated cleavage of peptides and proteins: An alternative to proteolytic digestion",
abstract = "Radical activated cleavage (RAC) is a new, virtually preparation-free method of protein and peptide fragmentation that produces a distinct pattern in less than 10 s. RAC of peptides uses hydroxyl radical production, at the surface of titanium dioxide (TiO2), to fragment the peptide backbone. Fragmentation of angiotensin I, lys-bradykinin and myoglobin was shown to be reproducible and rapid using RAC. Fragmentation occurred in predictable patterns suggesting cleavage of the peptide bond at proline. Additionally, enkephalin and Peptide A-779, two peptides that do not contain proline, showed no fragmentation under the same conditions. The peptide angiotensin was fragmented using several different device configurations including: (1) small volume wells; (2) microfluidic channels; and (3) a microflow reactor.",
keywords = "Cleavage, Fragmentation, Hydroxyl radical, Titanium dioxide",
author = "Jones, {Barbara J.} and Locascio, {Laurie E.} and Mark Hayes",
year = "2005",
month = jan,
day = "1",
language = "English (US)",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "286--288",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}