Batch mating experiments with Pseudomonas putida PAW 1 (TOL) as a donor and Pseudomonas aeruginosa PAO 1162 as a recipient strain were performed to quantify the effect of the substrate concentration in the mating medium on the observed plasmid transfer rate coefficient. The impact of the substrate concentration in the mating medium was highly correlated with the growth history of the donor strain. When the donor strain was harvested in exponential growth phase, no impact was observed; when the donor strain was taken from the stationary phase, however, a strong impact of the substrate concentration was measured: a 10‐fold reduction in the substrate concentration decreased the observed plasmid transfer rate by 55%.
|Number of pages
|Letters in Applied Microbiology
|Published - Sep 1995
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology