Proteomics of human urine

Visith Thongboonkerd; Pedro R. Cutillas; Robert J. Unwin; Stefan Schaub; Peter Nickerson; Marion Haubitz; Harald Mischak; Dobrin Nedelkov; Urban A. Kiernan; Randall W. Nelson

doi:10.1007/978-1-59745-432-2_11

Proteomics of human urine

Visith Thongboonkerd, Pedro R. Cutillas, Robert J. Unwin, Stefan Schaub, Peter Nickerson, Marion Haubitz, Harald Mischak, Dobrin Nedelkov, Urban A. Kiernan, Randall W. Nelson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Urine is one of the most interesting and useful human body fluids for clinical proteomics studies because of its availability in almost all patients and ease of collection without any invasive or painful procedure. Proteomic analysis of human urine offers invaluable information not only for a better understanding of the normal renal physiology and pathophysiology of kidney/kidney-related diseases, but also for biomarker discovery. This chapter provides principles, brief methods, and applications of various proteomic technologies (i.e., gel-based method, liquid chromatographybased approach, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, capillary electrophoresis coupled to mass spectrometry, and mass spectrometric immunoassay) to examine the human urine. At the end, perspectives and future directions in the field of urinary proteomics are discussed.

Original language	English (US)
Title of host publication	Proteomics of Human Body Fluids
Subtitle of host publication	Principles, Methods, and Applications
Publisher	Humana Press Inc.
Pages	225-268
Number of pages	44
ISBN (Electronic)	9781597454322
ISBN (Print)	9781588296573
DOIs	https://doi.org/10.1007/978-1-59745-432-2_11
State	Published - 2007

Keywords

2D-PAGE
SELDI
Urine
biomarker discovery
capillary electrophoresis
kidney
liquid chromatography
mass spectrometric immunoassay
pathophysiology
proteome

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/978-1-59745-432-2_11

Cite this

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abstract = "Urine is one of the most interesting and useful human body fluids for clinical proteomics studies because of its availability in almost all patients and ease of collection without any invasive or painful procedure. Proteomic analysis of human urine offers invaluable information not only for a better understanding of the normal renal physiology and pathophysiology of kidney/kidney-related diseases, but also for biomarker discovery. This chapter provides principles, brief methods, and applications of various proteomic technologies (i.e., gel-based method, liquid chromatographybased approach, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, capillary electrophoresis coupled to mass spectrometry, and mass spectrometric immunoassay) to examine the human urine. At the end, perspectives and future directions in the field of urinary proteomics are discussed.",

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AU - Thongboonkerd, Visith

AU - Cutillas, Pedro R.

AU - Unwin, Robert J.

AU - Schaub, Stefan

AU - Nickerson, Peter

AU - Haubitz, Marion

AU - Mischak, Harald

AU - Nedelkov, Dobrin

AU - Kiernan, Urban A.

AU - Nelson, Randall W.

PY - 2007

Y1 - 2007

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KW - 2D-PAGE

KW - SELDI

KW - Urine

KW - biomarker discovery

KW - capillary electrophoresis

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KW - liquid chromatography

KW - mass spectrometric immunoassay

KW - pathophysiology

KW - proteome

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BT - Proteomics of Human Body Fluids

PB - Humana Press Inc.

ER -

Proteomics of human urine

Abstract

Keywords

ASJC Scopus subject areas

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