@inbook{14ab0e4a57e1466aba2613d5a7434b23,
title = "Proteomic monitoring of B cell immunity",
abstract = "Immune monitoring is critical in settings of infection, autoimmunity, and cancer, but our understanding of the diversity of the antibody immune repertoire has been limited to selected target antigens and epitopes. Development of new vaccines requires monitoring of B cell immunity and identification of candidate antigens. As vaccines become more complex, novel techniques are required for monitoring the diversity of the B cell immune response. Since antibodies recognize both linear and conformational protein and glycoprotein epitopes, recent advances in proteomic and glycomic technologies for rapid display of antigenic structures are leading to methods for proteome-wide immune monitoring. Here, we review different approaches for protein display for immune monitoring, and provide methods for in situ protein display for the rapid detection and validation of antibody repertoires.",
keywords = "Antibody, B cell, Epitope, Immune monitoring, Immunity, Phage display, Protein arrays, Protein display",
author = "Radwa Ewaisha and Karen Anderson",
note = "Funding Information: This study was supported by NCI UOI CA11734 (K.S.A.). We thank Marika Hopper for assistance in the preparation of this manuscript and Julia Cheng for techinical assistance. Dr. Anderson is a consultant and has stock options with Provista Diagnostics Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = apr,
day = "1",
doi = "10.1007/978-1-4939-3387-7_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--152",
booktitle = "Methods in Molecular Biology",
}