Protein-RNA interactions in the subunits of human nuclear RNase P

Taijiao Jiang, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

Original languageEnglish (US)
Pages (from-to)937-941
Number of pages5
Issue number7
StatePublished - 2001
Externally publishedYes


  • HeLa cells
  • Precursor tRNAs
  • Three-hybrid assay
  • UV crosslinking

ASJC Scopus subject areas

  • Molecular Biology


Dive into the research topics of 'Protein-RNA interactions in the subunits of human nuclear RNase P'. Together they form a unique fingerprint.

Cite this