Programmable allosteric DNA regulations for molecular networks and nanomachines

Cheng Zhang, Xueying Ma, Xuedong Zheng, Yonggang Ke, Kuiting Chen, Dongsheng Liu, Zuhong Lu, Jing Yang, Hao Yan

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Structure-based molecular regulations have been widely adopted to modulate protein networks in cells and recently developed to control allosteric DNA operations in vitro. However, current examples of programmable allosteric signal transmission through integrated DNA networks are stringently constrained by specific design requirements. Developing a new, more general, and programmable scheme for establishing allosteric DNA networks remains challenging. Here, we developed a general strategy for programmable allosteric DNA regulations that can be finely tuned by varying the dimensions, positions, and number of conformational signals. By programming the allosteric signals, we realized fan-out/fan-in DNA gates and multiple-layer DNA cascading networks, as well as expanding the approach to long-range allosteric signal transmission through tunable DNA origami nanomachines ~100 nm in size. This strategy will enable programmable and complex allosteric DNA networks and nanodevices for nanoengineering, chemical, and biomedical applications displaying sense-compute-actuate molecular functionalities.

Original languageEnglish (US)
Article numbereabl4589
JournalScience Advances
Issue number5
StatePublished - Feb 2022

ASJC Scopus subject areas

  • General


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