Poly(acrylic acid) brushes pattern as a 3D functional biosensor surface for microchips

Yan Mei Wang, Yi Cui, Zhi Qiang Cheng, Lu Sheng Song, Zhi You Wang, Bao Hang Han, Jin Song Zhu

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Poly(acrylic acid) (PAA) brushes, a novel three dimensional (3D) precursor layer of biosensor or protein microarrays, possess high protein loading level and low non-specific protein adsorption. In this article, we describe a simple and convenient way to fabricate 3D PAA brushes pattern by microcontact printing (μCP) and characterize it with FT-IR and optical microscopy. The carboxyl groups of PAA brushes can be applied to covalently immobilize protein for immunoassay. Thriving 3D space made by patterning PAA brushes thin film is available to enhance protein immobilization, which is confirmed by measuring model protein interaction between human immunoglobulin G (H-IgG) and goat anti-H-IgG (G-H-IgG) with fluorescence microscopy and surface plasmon resonance imaging (SPRi). As expected, the SPRi signals of H-IgG coating on 3D PAA brushes pattern and further measuring specific binding with G-H-IgG are all larger than that of 3D PAA brushes without pattern and 2D bare gold surface. We further revealed that this surface can be used for high-throughput screening and clinical diagnosis by label-free assaying of Hepatitis-B-Virus surface antibody (HBsAb) with Hepatitis-B-Virus surface antigen (HBsAg) concentration array chip. The linearity range for HBsAb assay is wider than that of conventional ELISA method.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalApplied Surface Science
StatePublished - Feb 1 2013
Externally publishedYes


  • 3-Dimension
  • Hepatitis B virus
  • Microcontact printing
  • Poly(acrylic acid) brushes
  • Surface plasmon resonance imaging

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Surfaces and Interfaces


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