Pathogen reduction in human plasma using an ultrashort pulsed laser

Shaw Wei D Tsen, David H. Kingsley, Karen Kibler, Bertram Jacobs, Sara Sizemore, Sara Vaiana, Jeanne Anderson, Kong-Thon Tsen, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.

Original languageEnglish (US)
Article numbere111673
JournalPloS one
Issue number11
StatePublished - Nov 5 2014

ASJC Scopus subject areas

  • General


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