Abstract
We have reported the successful use of the Loop Mediated Isothermal Amplification (LAMP) reaction for Hepatitis B Virus (HBV) DNA in a 25 μl micro-reactor for the development of a rapid diagnostic device recently. This method shows the successful amplification of HBV DNA with high specificity and sensitivity efficiently within one hour. Such a powerful method can be used for many other fast diagnostic applications with the help of optimal primer design and miniaturization of reactor. In this report, we will first summarize the general design rules for primer design from literature reviews and our experimental results. We will discuss the effects of HBV primers and concentrations of working buffers for optimal performance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 137-144 |
| Number of pages | 8 |
| Journal | Biomedical Engineering - Applications, Basis and Communications |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2007 |
Keywords
- Hepatitis B virus
- Isothermal
- LAMP
- Primer design
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomedical Engineering
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