TY - JOUR
T1 - Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli
AU - Reed, Robin E.
AU - Baer, Madeline F.
AU - Guerrier-Takada, Cecilia
AU - Donis-Keller, Helen
AU - Altman, Sidney
N1 - Funding Information:
We are grateful to Ms. Isabel Pinto for excellent technical assistance and to Dr. M. Smith, Dr. H. Cudny and Mr. P. Furdon for advice. This research was supported by a US Public Health Service predoctoral training grant to R. R. and to grants from the US Public Health Service and National Science Foundation to S. A.
PY - 1982/9
Y1 - 1982/9
N2 - The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60% GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.
AB - The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60% GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.
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U2 - 10.1016/0092-8674(82)90259-8
DO - 10.1016/0092-8674(82)90259-8
M3 - Article
C2 - 6183002
AN - SCOPUS:0020184761
SN - 0092-8674
VL - 30
SP - 627
EP - 636
JO - Cell
JF - Cell
IS - 2
ER -