NMR Studies of Protein-Glycosaminoglycan Interactions

Research output: Chapter in Book/Report/Conference proceedingChapter


Glycosaminoglycans (GAGs) are a class of linear, sulfated polysaccharides. These extracellular polysaccharides interact with a range of protein partners and are active in controlling important biological phenomena, including reproduction, cell growth and differentiation, blood coagulation and immune system activation. As a result, interests in developing methods to control specific protein-GAG interactions are high. However, little high-resolution structural information on protein-GAG interactions is available, and predicting a protein's specificity for different GAG motifs remains challenging. Solution NMR has played crucial roles in analyzing specificity and dynamics of protein-GAG interactions. It is instrumental in determining GAG-binding sites of proteins and elucidating GAG-induced changes in protein dynamics. NMR's adaptability to GAG size and sulfation density means the technique can be used to investigate a large class of protein-GAG interactions even if homogeneous GAG samples are not available. In this chapter we will review some of the popular NMR techniques for studying protein-GAG interactions will be reviewed. Challenges in the study of protein-GAG systems and new techniques that may help in overcoming these challenges will also be examined.

Original languageEnglish (US)
Title of host publicationBiophysics and Biochemistry of Cartilage by NMR and MRI
EditorsWilliam S. Price, Koichi Kato, Thomas Peters
PublisherRoyal Society of Chemistry
Number of pages19
ISBN (Electronic)9781782621331, 9781782623106, 9781849736190
StatePublished - 2017

Publication series

NameNew Developments in NMR
ISSN (Print)2044-253X
ISSN (Electronic)2044-2548

ASJC Scopus subject areas

  • Analytical Chemistry
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy


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