Next-generation high-density self-assembling functional protein arrays

Niroshan Ramachandran; Jacob V. Raphael; Eugenie Hainsworth; Gokhan Demirkan; Manuel G. Fuentes; Andreas Rolfs; Yanhui Hu; Joshua LaBaer

doi:10.1038/nmeth.1210

Next-generation high-density self-assembling functional protein arrays

Niroshan Ramachandran, Jacob V. Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G. Fuentes, Andreas Rolfs, Yanhui Hu, Joshua LaBaer

Research output: Contribution to journal › Article › peer-review

268 Scopus citations

Abstract

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

Original language	English (US)
Pages (from-to)	535-538
Number of pages	4
Journal	Nature Methods
Volume	5
Issue number	6
DOIs	https://doi.org/10.1038/nmeth.1210
State	Published - Jun 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1210

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title = "Next-generation high-density self-assembling functional protein arrays",

abstract = "We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.",

author = "Niroshan Ramachandran and Raphael, {Jacob V.} and Eugenie Hainsworth and Gokhan Demirkan and Fuentes, {Manuel G.} and Andreas Rolfs and Yanhui Hu and Joshua LaBaer",

note = "Funding Information: We thank J. Williamson and M. Fernandez for their help with the robotics and D. Zhu and R. Boyce for developing the DNA normalization tool. This study was supported by the Early Detection Research Network (US National Cancer Institute grant 5U01CA117374-02) and the US National Institute of Allergy and Infectious Diseases (contract HHSN2332200400053C).",

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AU - Ramachandran, Niroshan

AU - Raphael, Jacob V.

AU - Hainsworth, Eugenie

AU - Demirkan, Gokhan

AU - Fuentes, Manuel G.

AU - Rolfs, Andreas

AU - Hu, Yanhui

AU - LaBaer, Joshua

N1 - Funding Information: We thank J. Williamson and M. Fernandez for their help with the robotics and D. Zhu and R. Boyce for developing the DNA normalization tool. This study was supported by the Early Detection Research Network (US National Cancer Institute grant 5U01CA117374-02) and the US National Institute of Allergy and Infectious Diseases (contract HHSN2332200400053C).

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Next-generation high-density self-assembling functional protein arrays

Abstract

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