TY - JOUR
T1 - New bimetallic porphyrin film
T2 - An electrocatalytic transducer for hydrogen peroxide reduction, applicable to first-generation oxidase-based biosensors
AU - Vago, Julio Miguel
AU - Dall'Orto, Viviana Campo
AU - Forzani, Erica
AU - Hurst, Jorge
AU - Rezzano, Irene Noemí
N1 - Funding Information:
Financial support from University of Buenos Aires (UBACyT B059), CONICET (641-PIP98), ANPCyT (Grant 06-06600 PICT99) are gratefully thanked. E. Forzani also thanks CONICET for a postdoctoral fellowship. The authors acknowledge to INQUIMAE and Professor Ernesto J. Calvo for EQCM and rotating disk measurements.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/11/15
Y1 - 2003/11/15
N2 - A bimetallic porphyrin film was studied as electrode modifier with catalytic activity for molecular oxygen reduction and hydrogen peroxide reduction. Molecular oxygen reduction is catalyzed by both polyFePPIX or polyCuPPIX. Peroxidase activity at-200 mV versus Ag/AgCl is only performed by polyFePPIX in absence of oxidative agents, and the deposit polyCuPPIX is required to perform peroxidase activity in the presence of oxygen. Copper substitution by other metals in the porphyrin was not effective. It has been found that metalloporphyrin immobilization order acquire particular relevance. However, the mass of porphyrin deposit is always 1.5-1.6 μg of polyFePPIX and 7.3-9.1 μg of polyCuPPIX, indicating that polymer growth rate is not affected by immobilization order. polyCuPPIX deposited on polyFePPIX preserves catalytic properties over a wide pH range, and acts as a molecular sieve. The use of this bimetallic electrode in a first-generation oxidase biosensors is discussed.
AB - A bimetallic porphyrin film was studied as electrode modifier with catalytic activity for molecular oxygen reduction and hydrogen peroxide reduction. Molecular oxygen reduction is catalyzed by both polyFePPIX or polyCuPPIX. Peroxidase activity at-200 mV versus Ag/AgCl is only performed by polyFePPIX in absence of oxidative agents, and the deposit polyCuPPIX is required to perform peroxidase activity in the presence of oxygen. Copper substitution by other metals in the porphyrin was not effective. It has been found that metalloporphyrin immobilization order acquire particular relevance. However, the mass of porphyrin deposit is always 1.5-1.6 μg of polyFePPIX and 7.3-9.1 μg of polyCuPPIX, indicating that polymer growth rate is not affected by immobilization order. polyCuPPIX deposited on polyFePPIX preserves catalytic properties over a wide pH range, and acts as a molecular sieve. The use of this bimetallic electrode in a first-generation oxidase biosensors is discussed.
KW - Bimetallic film
KW - Catalyst
KW - Electropolymerization
KW - Hydrogen peroxide
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U2 - 10.1016/S0925-4005(03)00575-6
DO - 10.1016/S0925-4005(03)00575-6
M3 - Article
AN - SCOPUS:0242571588
SN - 0925-4005
VL - 96
SP - 407
EP - 412
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-2
ER -