Model substrates for an RNA enzyme

William H. Mcclain; Cecilia Guerrier-Takada; Sidney Altman

doi:10.1126/science.2443980

Model substrates for an RNA enzyme

William H. Mcclain, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journal › Article › peer-review

239 Scopus citations

Abstract

M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3′ terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.

Original language	English (US)
Pages (from-to)	527-530
Number of pages	4
Journal	Science
Volume	238
Issue number	4826
DOIs	https://doi.org/10.1126/science.2443980
State	Published - 1987
Externally published	Yes

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title = "Model substrates for an RNA enzyme",

abstract = "M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3′ terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.",

author = "Mcclain, {William H.} and Cecilia Guerrier-Takada and Sidney Altman",

year = "1987",

doi = "10.1126/science.2443980",

language = "English (US)",

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pages = "527--530",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

number = "4826",

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T1 - Model substrates for an RNA enzyme

AU - Mcclain, William H.

AU - Guerrier-Takada, Cecilia

AU - Altman, Sidney

PY - 1987

Y1 - 1987

N2 - M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3′ terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.

AB - M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3′ terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.

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U2 - 10.1126/science.2443980

DO - 10.1126/science.2443980

M3 - Article

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AN - SCOPUS:0023663115

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VL - 238

SP - 527

EP - 530

JO - Science

JF - Science

IS - 4826

ER -

Model substrates for an RNA enzyme

Abstract

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