Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers

R. B. Doak, D. P. Deponte, G. Nelson, F. Camacho-Alanis, Alexandra Ros, John Spence, Uwe Weierstall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

Microscopic linear liquid free-streams offer a means of gently delivering biological samples into a probe beam in vacuum while maintaining the sample species in a fully solvated state. By employing gas dynamic forces to form the microscopic liquid stream (as opposed to a conventional solid-walled convergent nozzle), liquid free-streams down to 300 nm diameter have been generated. Such "Gas Dynamic Virtual Nozzles" (GDVN) are ideally suited to injecting complex biological species into an X-ray Free Electron Laser (XFEL) to determine the structure of the biological species via Serial Femtosecond Crystallography (SFX). GDVN injector technology developed for this purpose is described.

Original languageEnglish (US)
Title of host publication28th International Symposium on Rarefied Gas Dynamics 2012
Pages1314-1323
Number of pages10
Edition1
DOIs
StatePublished - 2012
Event28th International Symposium on Rarefied Gas Dynamics 2012, RGD 2012 - Zaragoza, Spain
Duration: Jul 9 2012Jul 13 2012

Publication series

NameAIP Conference Proceedings
Number1
Volume1501
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other28th International Symposium on Rarefied Gas Dynamics 2012, RGD 2012
Country/TerritorySpain
CityZaragoza
Period7/9/127/13/12

Keywords

  • Biological Structure Determination
  • Gas-Dynamic Virtual Nozzle
  • Nanocrystallography
  • Rayleigh Droplet Streams
  • Rayleigh-Plateau Instability
  • Serial Femtosecond Crystallography
  • X-ray Free Electron Laser

ASJC Scopus subject areas

  • General Physics and Astronomy

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