@article{1cd887f024d54ab390d2b10f48c6681f,
title = "Microcrystal electron diffraction methodology and applications",
abstract = "Microcrystal electron diffraction (MicroED) is a cryo-electron microscopy technique that utilizes three-dimensional nano- and microcrystals for high-resolution structure determination. These extremely small crystals are several orders of magnitude smaller than what is used in conventional x-ray diffraction experiments. MicroED is capable of providing high-quality data from samples that would otherwise be considered useless for diffraction measurements. Since its initial implementation, MicroED has been used in the fields of structural biology, chemistry, and materials science. In this article, we provide an overview of the MicroED methodology as well as examples of how MicroED in cryo-electron microscopy has been used for structure determination of a variety of samples.",
author = "Nannenga, {Brent L.} and Tamir Gonen",
note = "Funding Information: The Gonen laboratory is supported by funding from the Howard Hughes Medical Institute (HHMI). The Nannenga laboratory is supported by the National Institutes of Health (R01GM124152 and R21GM135784), and the Air Force Office of Scientific Research Grant No. FA9550-18-1-0012. MicroED was developed at the Janelia Research Campus of HHMI using HHMI funds and Janelia Visitor Program funds. Funding Information: The Gonen laboratory is supported by funding from the Howard Hughes Medical Institute (HHMI). The Nannenga laboratory is supported by the National Institutes of Health (R01GM124152 and R21GM135784), and the Air Force Office of Scientific Research Grant No. FA9550-18-1-0012. MicroED was developed at the Janelia Research Campus of HHMI using HHMI funds and Janelia Visitor Program funds. Brent Nannenga is an assistant professor of chemical engineering in the School for Engineering Matter, Transport and Energy at Arizona State University and a member of The Biodesign Institute{\textquoteright}s Center for Applied Structural Discovery. He obtained his PhD degree from the University of Washington in chemical engineering in 2011. His research focuses on developing and using methods for high-resolution structure determination, specifically microcrystal electron diffraction, and employing structural insights gained from these methods to engineer biomolecules and biological systems. His awards include an Air Force Office of Scientific Research Young Investigator Award, and a Fulton Outstanding Assistant Professor Award. Nannenga can be reached by email at brent.nannenga@asu.edu . Tamir Gonen is a professor of biological chemistry and physiology at the David Geffen School of Medicine of the University of California, Los Angeles, an investigator at the Howard Hughes Medical Institute, and a Member of the Royal Society of New Zealand. He obtained his PhD degree from The University of Auckland, New Zealand, in biological science, in 2002. In 2011, while leading a laboratory at the Howard Hughes Medical Institute, Janelia Research Campus, Gonen began developing microcrystal electron diffraction as a new method for structural biology. His research determined several previously unknown structures at resolutions better than 1 {\AA}. He has authored more than 100 publications and mentored faculty from top universities around the world. Gonen can be reached by email at tgonen@ucla.edu . Publisher Copyright: {\textcopyright} Materials Research Society 2019.",
year = "2019",
month = dec,
day = "1",
doi = "10.1557/mrs.2019.287",
language = "English (US)",
volume = "44",
pages = "956--960",
journal = "MRS Bulletin",
issn = "0883-7694",
publisher = "Materials Research Society",
number = "12",
}