Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Sarah J R Staton; Paul V. Jones; Ginger Ku; S. Douglass Gilman; Indu Kheterpal; Mark Hayes

doi:10.1039/c2an35138b

Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Sarah J R Staton
, Paul V. Jones
, Ginger Ku
, S. Douglass Gilman
, Indu Kheterpal
, Mark Hayes

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.

Original language	English (US)
Pages (from-to)	3227-3229
Number of pages	3
Journal	Analyst
Volume	137
Issue number	14
DOIs	https://doi.org/10.1039/c2an35138b
State	Published - Jul 21 2012

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/c2an35138b

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abstract = "Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400\%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.",

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AU - Kheterpal, Indu

AU - Hayes, Mark

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AB - Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.

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Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Abstract

ASJC Scopus subject areas

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