TY - JOUR
T1 - KINETICS OF PROTON-TRANSPORT-COUPLED ATP SYNTHESIS CATALYZED BY THE CHLOROPLAST ATP SYNTHASE.
AU - Graeber, P.
AU - Fromme, P.
AU - Junesch, U.
AU - Schmidt, G.
AU - Thulke, G.
PY - 1986/11/1
Y1 - 1986/11/1
N2 - The kinetics of proton-transport-coupled ATP synthesis catalyzed by the chloroplast ATPase (ATP synthase) was investigated after energization of the membrane by an artificially generated DELTA pH and an electric potential difference, DELTA psi . With a rapid mixing system, ATP synthesis was studied at short reaction times ( less than 200 ms) where all relevant parameters (pH//o//u//t, pH//i//n, DELTA psi , substrate and product concentrations) remain practically constant at their initial values. The reaction is investigated at three levels of complexity. (1) The ATPase is embedded in the natural membrane. (2) The ATPase is isolated, purified and reconstituted into asolectin liposomes. (3) The reconstituted ATPase is investigated under single site, single turnover conditions.
AB - The kinetics of proton-transport-coupled ATP synthesis catalyzed by the chloroplast ATPase (ATP synthase) was investigated after energization of the membrane by an artificially generated DELTA pH and an electric potential difference, DELTA psi . With a rapid mixing system, ATP synthesis was studied at short reaction times ( less than 200 ms) where all relevant parameters (pH//o//u//t, pH//i//n, DELTA psi , substrate and product concentrations) remain practically constant at their initial values. The reaction is investigated at three levels of complexity. (1) The ATPase is embedded in the natural membrane. (2) The ATPase is isolated, purified and reconstituted into asolectin liposomes. (3) The reconstituted ATPase is investigated under single site, single turnover conditions.
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M3 - Article
AN - SCOPUS:0022812109
SN - 0005-9021
VL - 11
SP - 1034
EP - 1040
JO - Berichte der Bunsengesellschaft/Physical Chemistry Chemical Physics
JF - Berichte der Bunsengesellschaft/Physical Chemistry Chemical Physics
IS - 90
ER -