Kinetic biomineralization through microfluidic chip tests

Yang Xiao, Xiang He, Wei Wu, Armin W. Stuedlein, T. Matthew Evans, Jian Chu, Hanlong Liu, Leon A. van Paassen, Huanran Wu

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


A homogeneous microfluidic chip was used to investigate the pore-scale characteristics during the process of microbially induced calcium carbonate precipitation (MICP). An image-processing scheme was developed to measure the projecting areas of the precipitated calcium carbonate. Calcium carbonate first precipitated on the bacterium side before spreading to the rest of the chip. The distribution of calcium carbonate was more uniform along the length of the microchip than along the width. Raman back-scattering spectroscopy was used to examine the chemical composition of the precipitate, identifying calcite and vaterite as the main mineral phases. Bacterium traces were noted on crystal surfaces in SEM images, suggesting a higher adsorptive capacity for irregular precipitates than well-shaped crystals.

Original languageEnglish (US)
Pages (from-to)3229-3237
Number of pages9
JournalActa Geotechnica
Issue number10
StatePublished - Oct 2021
Externally publishedYes


  • MICP
  • Microfluidic chip
  • Mineral phase
  • Porous media

ASJC Scopus subject areas

  • Geotechnical Engineering and Engineering Geology
  • Earth and Planetary Sciences (miscellaneous)


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