TY - CHAP
T1 - Isotope labeling in insect cells
AU - Saxena, Krishna
AU - Dutta, Arpana
AU - Klein-Seetharaman, Judith
AU - Schwalbe, Harald
PY - 2012
Y1 - 2012
N2 - Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.
AB - Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.
KW - Baculovirus
KW - Insect cells
KW - Isotope labeling
KW - Nuclear magnetic resonance
KW - Recombinant protein expression
UR - http://www.scopus.com/inward/record.url?scp=84855902846&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855902846&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-480-3_3
DO - 10.1007/978-1-61779-480-3_3
M3 - Chapter
C2 - 22167667
AN - SCOPUS:84855902846
SN - 9781617794797
T3 - Methods in Molecular Biology
SP - 37
EP - 54
BT - Protein NMR Techniques
ER -