Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4

George Pettit; S. B. Singh; E. Hamel; C. M. Lin; D. S. Alberts; D. Garcia-Kendal

doi:10.1007/BF01954881

Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4

George Pettit, S. B. Singh, E. Hamel, C. M. Lin, D. S. Alberts, D. Garcia-Kendal

Research output: Contribution to journal › Article › peer-review

769 Scopus citations

Abstract

The African tree Combretum caffrum (Combretaceae) has been found to contain a powerful inhibitor of tubulin polymerization (IC₅₀2-3 μM), the growth of murine lymphocytic leukemia (L 1210 and P 388 with ED₅₀≈0.003 μM and human colon cancer cell lines [(e.g. LoVo (ED₅₀=0.005 μg/ml), HT29 (ED₅₀ 0.02 μg/ml, Colo 205 (ED₅₀=0.07 μg/ml), DLD-1 (ED₅₀=0.005 μg/ml) and HCT-15 (ED₅₀=0.0009 μg/ml)] designated combretastatin A-4 (1c). The structure assigned by spectral techniques was confirmed by synthesis.

Original language	English (US)
Pages (from-to)	209-211
Number of pages	3
Journal	Experientia
Volume	45
Issue number	2
DOIs	https://doi.org/10.1007/BF01954881
State	Published - Feb 1989

Keywords

Combretum caffrum
combretaceae
combretastatin A-4
human colon cancer
inhibitor tubulin
lymphocytic leukemia

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/BF01954881

Cite this

@article{01842cbcd8c941c2bb029041745f0fa6,

title = "Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4",

abstract = "The African tree Combretum caffrum (Combretaceae) has been found to contain a powerful inhibitor of tubulin polymerization (IC502-3 μM), the growth of murine lymphocytic leukemia (L 1210 and P 388 with ED50≈0.003 μM and human colon cancer cell lines [(e.g. LoVo (ED50=0.005 μg/ml), HT29 (ED50 0.02 μg/ml, Colo 205 (ED50=0.07 μg/ml), DLD-1 (ED50=0.005 μg/ml) and HCT-15 (ED50=0.0009 μg/ml)] designated combretastatin A-4 (1c). The structure assigned by spectral techniques was confirmed by synthesis.",

keywords = "Combretum caffrum, combretaceae, combretastatin A-4, human colon cancer, inhibitor tubulin, lymphocytic leukemia",

author = "George Pettit and Singh, {S. B.} and E. Hamel and Lin, {C. M.} and Alberts, {D. S.} and D. Garcia-Kendal",

year = "1989",

month = feb,

doi = "10.1007/BF01954881",

language = "English (US)",

volume = "45",

pages = "209--211",

journal = "Experientia",

issn = "0014-4754",

publisher = "SAGE Publications Ltd",

number = "2",

}

TY - JOUR

T1 - Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4

AU - Pettit, George

AU - Singh, S. B.

AU - Hamel, E.

AU - Lin, C. M.

AU - Alberts, D. S.

AU - Garcia-Kendal, D.

PY - 1989/2

Y1 - 1989/2

N2 - The African tree Combretum caffrum (Combretaceae) has been found to contain a powerful inhibitor of tubulin polymerization (IC502-3 μM), the growth of murine lymphocytic leukemia (L 1210 and P 388 with ED50≈0.003 μM and human colon cancer cell lines [(e.g. LoVo (ED50=0.005 μg/ml), HT29 (ED50 0.02 μg/ml, Colo 205 (ED50=0.07 μg/ml), DLD-1 (ED50=0.005 μg/ml) and HCT-15 (ED50=0.0009 μg/ml)] designated combretastatin A-4 (1c). The structure assigned by spectral techniques was confirmed by synthesis.

AB - The African tree Combretum caffrum (Combretaceae) has been found to contain a powerful inhibitor of tubulin polymerization (IC502-3 μM), the growth of murine lymphocytic leukemia (L 1210 and P 388 with ED50≈0.003 μM and human colon cancer cell lines [(e.g. LoVo (ED50=0.005 μg/ml), HT29 (ED50 0.02 μg/ml, Colo 205 (ED50=0.07 μg/ml), DLD-1 (ED50=0.005 μg/ml) and HCT-15 (ED50=0.0009 μg/ml)] designated combretastatin A-4 (1c). The structure assigned by spectral techniques was confirmed by synthesis.

KW - Combretum caffrum

KW - combretaceae

KW - combretastatin A-4

KW - human colon cancer

KW - inhibitor tubulin

KW - lymphocytic leukemia

UR - http://www.scopus.com/inward/record.url?scp=0024513175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024513175&partnerID=8YFLogxK

U2 - 10.1007/BF01954881

DO - 10.1007/BF01954881

M3 - Article

C2 - 2920809

AN - SCOPUS:0024513175

SN - 0014-4754

VL - 45

SP - 209

EP - 211

JO - Experientia

JF - Experientia

IS - 2

ER -

Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this