TY - JOUR
T1 - Isolation and purification of the ovulation-inducing factor from seminal plasma in the bactrian camel (Camelus bactrianus)
AU - Pan, G.
AU - Chen, Z.
AU - Liu, X.
AU - Li, D.
AU - Xie, Q.
AU - Ling, F.
AU - Fang, L.
N1 - Funding Information:
Acknowledgments This research was sponsored by grants from the China National Natural Science Foundation (Numbers 39270534 and 39670539) from 1993 to 1999. The authors thank Prof. G. Pan, Arizona State University; B.X. Chen, Gansu Agricultural University; S. Xu, Beijing Biophysics; G. Wan, B. Xin, Beijing Chemistry Institute of Chinese Academy Sciences; and Y. Gong, Shanghai Biochemistry Institute of Chinese Academy Sciences. ’ Correspondence and reprint requests, e-mail: [email protected].
PY - 2001/6/1
Y1 - 2001/6/1
N2 - The purpose of this study was to extract, identify and partially characterize a newly found ovulation-inducing factor, and thus gain our understanding of induced ovulation in biology. In our preliminary research, an ovulation-inducing factor (OIF) was isolated and purified from seminal plasma of the bactrian camel by ion-exchange chromatography on DEAE-cellulose, HPLC and reverse-phase HPLC. The OIF is a peptide with 74 residues and GnRH-like bioactivity, which is heat-stable in camel seminal plasma because the OIF is wrapped up in several protein layers with different properties. However, purified OIF is degraded when it is exposed to oxygen or is heated in water. According to the analysis of amino acid components and partial amino acid sequence aminated N-terminus, and its molecular weight, the OIF is completely different from the native-LHRH, LH, HCG, PMSG and PGF-2 α. The OIF is a novel ovulation hormone in the bactrian camel, but is similar to that reported in the bull.
AB - The purpose of this study was to extract, identify and partially characterize a newly found ovulation-inducing factor, and thus gain our understanding of induced ovulation in biology. In our preliminary research, an ovulation-inducing factor (OIF) was isolated and purified from seminal plasma of the bactrian camel by ion-exchange chromatography on DEAE-cellulose, HPLC and reverse-phase HPLC. The OIF is a peptide with 74 residues and GnRH-like bioactivity, which is heat-stable in camel seminal plasma because the OIF is wrapped up in several protein layers with different properties. However, purified OIF is degraded when it is exposed to oxygen or is heated in water. According to the analysis of amino acid components and partial amino acid sequence aminated N-terminus, and its molecular weight, the OIF is completely different from the native-LHRH, LH, HCG, PMSG and PGF-2 α. The OIF is a novel ovulation hormone in the bactrian camel, but is similar to that reported in the bull.
KW - Bactrian camel
KW - Isolation
KW - Ovulation-inducing factor
KW - Purification
KW - Seminal plasma
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U2 - 10.1016/S0093-691X(01)00528-3
DO - 10.1016/S0093-691X(01)00528-3
M3 - Article
C2 - 11414491
AN - SCOPUS:0035367285
SN - 0093-691X
VL - 55
SP - 1863
EP - 1879
JO - Theriogenology
JF - Theriogenology
IS - 9
ER -