In vivo protein crystallization opens new routes in structural biology

Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P. Deponte, Thomas A. White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, Sébastien Boutet, John D. Bozek, Carl Caleman, Nicola CoppolaJan Davidsson, R. Bruce Doak, Tomas Ekeberg, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Gülnter Hauser, Helmut Hirsemann, Peter Holl, Mark S. Hunter, Stephan Kassemeyer, Richard Kirian, Lukas Lomb, Filipe R N C Maia, Nils Kimmel, Andrew V. Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond G. Sierra, Heike Soltau, Stephan Stern, Lothar Strülder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J. Williams, Cornelia B. Wunderer, Petra Fromme, John Spence, Thilo Stehle, Henry N. Chapman, Christian Betzel, Michael Duszenko

Research output: Contribution to journalArticlepeer-review

147 Scopus citations


Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Original languageEnglish (US)
Pages (from-to)259-262
Number of pages4
JournalNature Methods
Issue number3
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'In vivo protein crystallization opens new routes in structural biology'. Together they form a unique fingerprint.
  • Crystal structure of Cathepsin B from Trypanosoma Brucei

    Koopmann, R. (Contributor), Cupelli, K. (Contributor), Redecke, L. (Contributor), Nass, K. (Contributor), Deponte, D. P. (Contributor), White, T. A. (Contributor), Stellato, F. (Contributor), Rehders, D. (Contributor), Liang, M. (Contributor), Andreasson, J. (Contributor), Aquila, A. (Contributor), Bajt, S. (Contributor), Barthelmess, M. (Contributor), Barty, A. (Contributor), Bogan, M. J. (Contributor), Bostedt, C. (Contributor), Boutet, S. (Contributor), Bozek, J. D. (Contributor), Caleman, C. (Contributor), Coppola, N. (Contributor), Davidsson, J. (Contributor), Doak, R. B. (Contributor), Ekeberg, T. (Contributor), Epp, S. W. (Contributor), Erk, B. (Contributor), Fleckenstein, H. (Contributor), Foucar, L. (Contributor), Graafsma, H. (Contributor), Gumprecht, L. (Contributor), Hajdu, J. (Contributor), Hampton, C. Y. (Contributor), Hartmann, A. (Contributor), Hartmann, R. (Contributor), Hauser, G. (Contributor), Hirsemann, H. (Contributor), Holl, P. (Contributor), Hunter, M. S. (Contributor), Kassemeyer, S. (Contributor), Kirian, R. (Contributor), Lomb, L. (Contributor), Maia, F. R. N. C. (Contributor), Kimmel, N. (Contributor), Martin, A. V. (Contributor), Messerschmidt, M. (Contributor), Reich, C. (Contributor), Rolles, D. (Contributor), Rudek, B. (Contributor), Rudenko, A. (Contributor), Schlichting, I. (Contributor), Schulz, J. (Contributor), Seibert, M. M. (Contributor), Shoeman, R. L. (Contributor), Sierra, R. G. (Contributor), Soltau, H. (Contributor), Stern, S. (Contributor), Strülder, L. (Contributor), Timneanu, N. (Contributor), Ullrich, J. (Contributor), Wang, X. (Contributor), Weidenspointner, G. (Contributor), Weierstall, U. (Contributor), Williams, G. J. (Contributor), Wunderer, C. B. (Contributor), Fromme, P. (Contributor), Spence, J. C. H. (Contributor), Stehle, T. (Contributor), Chapman, H. N. (Contributor), Betzel, C. (Contributor) & Duszenko, M. (Contributor), Protein Data Bank (PDB), Nov 2 2011


Cite this