IFN-γ upregulation and protection by macrophage-adapted infectious bursal disease virus

Mahesh Khatri, Jagdev M. Sharma

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Infectious bursal disease virus (IBDV) causes an acute, highly contagious and immunosuppressive disease in chickens. The virus infects and destroys actively dividing IgM-bearing B cells in the bursa. Although antibody response is considered important in defense against virulent IBDV, antibody alone is not sufficient and cell-mediated immunity (CMI) appears to play a critical role. We serially passaged classical IBDV (cIBDV) in MQ-NCSU, an avian macrophage cell line. The macrophage-adapted virus (mcIBDV) was used in ovo to immunize chickens. mcIBDV, which was non-pathogenic and highly protective, induced anti-IBDV antibody and, most importantly, upregulated the expression of IFN-γ mRNA in spleen. The IFN-γ upregulation by mcIBDV was significantly higher (P < 0.05) than that induced by a commercially available vaccine originated from adaptation of cIBDV in the tissue culture (chicken embryo fibroblast (CEF) cells) (tcIBDV). The level of IFN-γ upregulation by cIBDV that had been adapted by serial passages to CEF (fcIBDV) was similar to that by tcIBDV and significantly lower (P < 0.05) than that by mcIBDV. Virus load was significantly higher (P < 0.05) in spleen macrophages obtained from mcIBDV-inoculated chickens than that from fcIBDV-inoculated chickens. These data indicated that adaptation of IBDV to macrophages enhanced the ability of the virus to induce cell-mediated immune response in chickens.

Original languageEnglish (US)
Pages (from-to)4740-4746
Number of pages7
Issue number36
StatePublished - Aug 26 2008


  • Cell-mediated immunity
  • IBDV
  • Protection

ASJC Scopus subject areas

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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